ABSTRACT
Viral production and transduction have seen a considerable increase in use for gene therapies and especially vaccines (e.g., COVID-19) due to their abilities to deliver a significant amount of genetic information and integrate it into the genome. However, challenges associated with viral production/transduction involve steps that are very time consuming, manually intensive, and laborious. In efforts to expedite this process, we have created a microfluidic methodology that will provide a “hands-off” workflow in the genome engineering pipeline. In this work, we developed a platform which can generate lentiviral particles on-demand containing the gene-editing machinery that will be able to modify target breast cancer cell lines. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
ABSTRACT
INTRODUCTION AND OBJECTIVE: Spina Bifida (SB) is a congenital spinal defect with multiple urologic manifestations requiring life-long urologic care. Transition to adult health care can be particularly difficult for patients with complex medical issues, especially during COVID-19. Our team created an online educational series for patients with SB to help provide education and community building. Herein, we describe our experience with a virtual SB Education Series and the results of survey-based patient experience through a virtual platform. METHODS: Patients with SB ages 16-24 and their caregivers were identified at a single tertiary academic medical center and were invited to the zoom education day series. Pre and post session surveys were collected, and data analyzed. RESULTS: There were a total of 68 registrations for 4 conferences held between 5/3/20-12/9/20. 24, 24, 21, 36 invitations were sent out before each session and 18,18, 11, 21 patients attended each education session, respectively. A total of 46% (31/68) participants completed the pre-survey and 19% (13/68) completed the post-survey. The survey results can be found in Table 1. Participants were comfortable using telehealth for medical visits (4.87), found them easy (4.69) and would use telehealth for future appointments (4.7). This did not increase significantly after the educational sessions. However, there was a significant increase in patients' comfort of navigating non-emergent medical problems during the COVID-19 pandemic between pre and post-survey results (4.07 vs 5.17, p <0.01). Importantly, participants did not feel that they lost the ability to connect with other individuals during the pandemic (4.45) and were strongly in favor of attending future conferences. CONCLUSIONS: This program aimed to reach patients with SB who were transitioning from pediatric to adult urology. Attendance increased with higher volume of direct outreach of patients in the adult and pediatric SB clinics. Importantly, by engaging in online educational series, participants became more comfortable managing non-emergent urologic issues. Transitional urology patients with SB can benefit from online educational sessions and this platform was well received. Future directions will be to increase participants and determine the effectiveness of video educational session on participants' access to care.
ABSTRACT
The suspension of the 2019–2020 National Basketball Association (NBA) season due to the COVID-19 pandemic meant that NBA fans were unable to engage in an activity that they loved in the midst of a global health crisis. In this research, we assessed if fan responses to the suspension were associated with different types of fan passion and motives. Shortly after the NBA season suspension, NBA fans (N = 395) completed online surveys assessing harmonious and obsessive passion for being an NBA fan, motives for watching games, and various attitudes and responses to the suspension. We found that both fan passion and motives predicted responses to the suspension, particularly obsessive passion which predicted greater levels of distress, coping responses, and negative attitudes toward the suspension. These findings have implications for both the passion and fan motives literatures. © 2021 Elsevier Ltd